7EOQ

Structure of the human GluN1/GluN2A NMDA receptor in the glycine/CPP bound state

Summary for 7EOQ

• Entry DOI: 10.2210/pdb7eoq/pdb
• EMDB information: 31227
• Descriptor: Glutamate receptor ionotropic, NMDA 2A, Glutamate receptor ionotropic, NMDA 1, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: nmda receptor, membrane protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 383769.68

Authors

Wang, H.,Zhu, S. (deposition date: 2021-04-22, release date: 2021-06-30, Last modification date: 2024-10-30)

Primary citation

Wang, H.,Lv, S.,Stroebel, D.,Zhang, J.,Pan, Y.,Huang, X.,Zhang, X.,Paoletti, P.,Zhu, S.
Gating mechanism and a modulatory niche of human GluN1-GluN2A NMDA receptors.
Neuron, 109:2443-2456.e5, 2021

PubMed Abstract: N-methyl-D-aspartate (NMDA) receptors are glutamate-gated calcium-permeable ion channels that are widely implicated in synaptic transmission and plasticity. Here, we report a gallery of cryo-electron microscopy (cryo-EM) structures of the human GluN1-GluN2A NMDA receptor at an overall resolution of 4 Å in complex with distinct ligands or modulators. In the full-length context of GluN1-GluN2A receptors, we visualize the competitive antagonists bound to the ligand-binding domains (LBDs) of GluN1 and GluN2A subunits, respectively. We reveal that the binding of positive allosteric modulator shortens the distance between LBDs and the transmembrane domain (TMD), which further stretches the opening of the gate. In addition, we unexpectedly visualize the binding cavity of the "foot-in-the-door" blocker 9-aminoacridine within the LBD-TMD linker region, differing from the conventional "trapping" blocker binding site at the vestibule within the TMD. Our study provides molecular insights into the crosstalk between LBDs and TMD during channel activation, inhibition, and allosteric transition.

PubMed: 34186027
DOI: 10.1016/j.neuron.2021.05.031

Experimental method

ELECTRON MICROSCOPY (4.1 Å)