7EKD

Crystal structure of gibberellin 3-oxidase 2 (GA3ox2) in rice

7EKD の概要

• エントリーDOI: 10.2210/pdb7ekd/pdb
• 分子名称: Gibberellin 3-beta-dioxygenase 2, PHOSPHATE ION, 2-OXOGLUTARIC ACID, ... (6 entities in total)
• 機能のキーワード: 2-oxoglutarate-dependent dioxygenase, biosynthetic protein
• 由来する生物種: Oryza sativa subsp. japonica (Rice)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41586.71

構造登録者

Takehara, S.,Kawai, K.,Mikami, B.,Ueguchi-Tanaka, M. (登録日: 2021-04-05, 公開日: 2022-02-16, 最終更新日: 2023-11-29)

主引用文献

Kawai, K.,Takehara, S.,Kashio, T.,Morii, M.,Sugihara, A.,Yoshimura, H.,Ito, A.,Hattori, M.,Toda, Y.,Kojima, M.,Takebayashi, Y.,Furuumi, H.,Nonomura, K.I.,Mikami, B.,Akagi, T.,Sakakibara, H.,Kitano, H.,Matsuoka, M.,Ueguchi-Tanaka, M.
Evolutionary alterations in gene expression and enzymatic activities of gibberellin 3-oxidase 1 in Oryza.
Commun Biol, 5:67-67, 2022

PubMed Abstract: Proper anther and pollen development are important for plant reproduction. The plant hormone gibberellin is important for anther development in rice, but its gametophytic functions remain largely unknown. Here, we report the functional and evolutionary analyses of rice gibberellin 3-oxidase 1 (OsGA3ox1), a gibberellin synthetic enzyme specifically expressed in the late developmental stages of anthers. Enzymatic and X-ray crystallography analyses reveal that OsGA3ox1 has a higher GA synthesis ratio than OsGA3ox2. In addition, we generate an osga3ox1 knockout mutant by genome editing and demonstrate the bioactive gibberellic acid synthesis by the OsGA3ox1 action during starch accumulation in pollen via invertase regulation. Furthermore, we analyze the evolution of Oryza GA3ox1s and reveal that their enzyme activity and gene expression have evolved in a way that is characteristic of the Oryza genus and contribute to their male reproduction ability.

PubMed: 35046494
DOI: 10.1038/s42003-022-03008-5

実験手法

X-RAY DIFFRACTION (1.899 Å)