7EJS

Structure of ERH-2 bound to PICS-1

7EJS の概要

• エントリーDOI: 10.2210/pdb7ejs/pdb
• 分子名称: Enhancer of rudimentary homolog 2,Protein pid-3 (2 entities in total)
• 機能のキーワード: pics complex, peptide binding protein
• 由来する生物種: Caenorhabditis elegans; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31885.23

構造登録者

Wang, X.,Xu, C. (登録日: 2021-04-02, 公開日: 2021-08-25, 最終更新日: 2023-11-29)

主引用文献

Wang, X.,Zeng, C.,Liao, S.,Zhu, Z.,Zhang, J.,Tu, X.,Yao, X.,Feng, X.,Guang, S.,Xu, C.
Molecular basis for PICS-mediated piRNA biogenesis and cell division.
Nat Commun, 12:5595-5595, 2021

PubMed Abstract: By incorporating two mutually exclusive factors, PID-1 and TOST-1, C. elegans PICS complex plays important roles in piRNA biogenesis, chromosome segregation and cell division. We firstly map the interaction network between PICS subunits, then uncover the mechanisms underlying the interactions between PICS subunits by solving several complex structures, including those of TOFU-6/PICS-1, ERH-2/PICS-1, and ERH-2/TOST-1. Our biochemical experiment also demonstrates that PICS exists as an octamer consisting of two copies of each subunit. Combining structural analyses with mutagenesis experiments, we identify interfacial residues of PICS subunits that are critical for maintaining intact PICS complex in vitro. Furthermore, using genetics, cell biology and imaging experiments, we find that those mutants impairing the in vitro interaction network within PICS, also lead to dysfunction of PICS in vivo, including mislocalization of PICS, and reduced levels of piRNAs or aberrant chromosome segregation and cell division. Therefore, our work provides structural insights into understanding the PICS-mediated piRNA biogenesis and cell division.

PubMed: 34552083
DOI: 10.1038/s41467-021-25896-7

実験手法

X-RAY DIFFRACTION (2.387 Å)