7EJM

Complex Structure of HLA-A*2402 with the Peptide from HCoV(CoV-229E) spike protein

7EJM の概要

• エントリーDOI: 10.2210/pdb7ejm/pdb
• 分子名称: MHC class I antigen, Beta-2-microglobulin, 9-mer peptide from the HCoV spike protein, ... (4 entities in total)
• 機能のキーワード: hla-a*2402, mhc class i, immune system, coronavirus
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45618.49

構造登録者

Murayama, K.,Kato-Murayama, M.,Shirouzu, M. (登録日: 2021-04-02, 公開日: 2022-01-26, 最終更新日: 2024-11-06)

主引用文献

Shimizu, K.,Iyoda, T.,Sanpei, A.,Nakazato, H.,Okada, M.,Ueda, S.,Kato-Murayama, M.,Murayama, K.,Shirouzu, M.,Harada, N.,Hidaka, M.,Fujii, S.I.
Identification of TCR repertoires in functionally competent cytotoxic T cells cross-reactive to SARS-CoV-2.
Commun Biol, 4:1365-1365, 2021

PubMed Abstract: SARS-CoV-2-specific CD8 T cells are scarce but detectable in unexposed healthy donors (UHDs). It remains unclear whether pre-existing human coronavirus (HCoV)-specific CD8 T cells are converted to functionally competent T cells cross-reactive to SARS-CoV-2. Here, we identified the HLA-A24-high binding, immunodominant epitopes in SARS-CoV-2 spike region that can be recognized by seasonal coronavirus-specific CD8 T cells from HLA-A24 UHDs. Cross-reactive CD8 T cells were clearly reduced in patients with hematological malignancy, who are usually immunosuppressed, compared to those in UHDs. Furthermore, we showed that CD8 T cells in response to a selected dominant epitope display multifunctionality and cross-functionality across HCoVs in HLA-A24 donors. Cross-reactivity of T-cell receptors isolated from them exhibited selective diversity at the single-cell level. Taken together, when stimulated well by immunodominant epitopes, selective pre-existing CD8 T cells with high functional avidity may be cross-reactive against SARS-CoV-2.

PubMed: 34857854
DOI: 10.1038/s42003-021-02885-6

実験手法

X-RAY DIFFRACTION (1.71 Å)