7EHO

Chitin oligosaccharide binding protein

7EHO の概要

• エントリーDOI: 10.2210/pdb7eho/pdb
• 分子名称: Chitin oligosaccharide binding protein NagB2, TETRAETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: chitin oligosaccharide binding protein, sugar binding protein
• 由来する生物種: Paenibacillus sp. FPU-7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94844.91

構造登録者

Itoh, T.,Hibi, T.,Kimoto, H. (登録日: 2021-03-30, 公開日: 2021-07-07, 最終更新日: 2024-05-29)

主引用文献

Itoh, T.,Yaguchi, M.,Nakaichi, A.,Yoda, M.,Hibi, T.,Kimoto, H.
Structural characterization of two solute-binding proteins for N,N' -diacetylchitobiose/ N,N',N'' -triacetylchitotoriose of the gram-positive bacterium, Paenibacillus sp. str. FPU-7.
J Struct Biol X, 5:100049-100049, 2021

PubMed Abstract: The chitinolytic bacterium sp. str. FPU-7 efficiently degrades chitin into oligosaccharides such as -acetyl-D-glucosamine (GlcNAc) and disaccharides (GlcNAc) through multiple secretory chitinases. Transport of these oligosaccharides by . str. FPU-7 has not yet been clarified. In this study, we identified , predicted to encode a sugar solute-binding protein (SBP), which is a component of the ABC transport system. However, the genes next to were predicted to encode two-component regulatory system proteins rather than transmembrane domains (TMDs). We also identified , which is highly homologous to . Adjacent to , two genes were predicted to encode TMDs. Binding experiments of the recombinant NagB1 and NagB2 to several oligosaccharides using differential scanning fluorimetry and surface plasmon resonance confirmed that both proteins are SBPs of (GlcNAc) and (GlcNAc). We determined their crystal structures complexed with and without chitin oligosaccharides at a resolution of 1.2 to 2.0 Å. The structures shared typical SBP structural folds and were classified as subcluster D-I. Large domain motions were observed in the structures, suggesting that they were induced by ligand binding via the "Venus flytrap" mechanism. These structures also revealed chitin oligosaccharide recognition mechanisms. In conclusion, our study provides insight into the recognition and transport of chitin oligosaccharides in bacteria.

PubMed: 34195603
DOI: 10.1016/j.yjsbx.2021.100049

実験手法

X-RAY DIFFRACTION (1.79 Å)