7EH3

Structure of Nanobody Nb23 in solution using NMR spectroscopy

7EH3 の概要

• エントリーDOI: 10.2210/pdb7eh3/pdb
• 分子名称: Nb23 (1 entity in total)
• 機能のキーワード: single-domain antibody, antibody fragment, immune system
• 由来する生物種: Lama glama
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15214.81

構造登録者

Percipalle, M.,Hunashal, Y.,Esposito, G.,Fogolari, F. (登録日: 2021-03-28, 公開日: 2021-07-14, 最終更新日: 2024-11-20)

主引用文献

Percipalle, M.,Hunashal, Y.,Steyaert, J.,Fogolari, F.,Esposito, G.
Structure of Nanobody Nb23.
Molecules, 26:-, 2021

PubMed Abstract: Nanobodies, or VHHs, are derived from heavy chain-only antibodies (hcAbs) found in camelids. They overcome some of the inherent limitations of monoclonal antibodies (mAbs) and derivatives thereof, due to their smaller molecular size and higher stability, and thus present an alternative to mAbs for therapeutic use. Two nanobodies, Nb23 and Nb24, have been shown to similarly inhibit the self-aggregation of very amyloidogenic variants of β2-microglobulin. Here, the structure of Nb23 was modeled with the Chemical-Shift (CS)-Rosetta server using chemical shift assignments from nuclear magnetic resonance (NMR) spectroscopy experiments, and used as prior knowledge in PONDEROSA restrained modeling based on experimentally assessed internuclear distances. Further validation was comparatively obtained with the results of molecular dynamics trajectories calculated from the resulting best energy-minimized Nb23 conformers.

PubMed: 34207949
DOI: 10.3390/molecules26123567

実験手法

SOLUTION NMR