7EFY

Crystal structure of retroviral protease-like domain of Ddi1 from Cryptosporidium hominis

Summary for 7EFY

• Entry DOI: 10.2210/pdb7efy/pdb
• Descriptor: UBA domain-containing protein (2 entities in total)
• Functional Keywords: aspartic protease, hydrolase
• Biological source: Cryptosporidium hominis
• Total number of polymer chains: 1
• Total formula weight: 14245.47

Authors

Biswas, I.B.,Killivalavan, A.K.,Suguna, K.S. (deposition date: 2021-03-23, release date: 2022-02-09, Last modification date: 2023-11-29)

Primary citation

Asaithambi, K.,Biswas, I.,Suguna, K.
Structural and functional insights into the DNA damage-inducible protein 1 (Ddi1) from protozoa.
Curr Res Struct Biol, 4:175-191, 2022

PubMed Abstract: Ddi1 is a multidomain protein that belongs to the ubiquitin receptor family of proteins. The Ddi1 proteins contain a highly conserved retroviral protease (RVP)-like domain along with other domains. The severity of opportunistic infections, caused by parasitic protozoa in AIDS patients, was found to decline when HIV protease inhibitors were used in antiretroviral therapy. Parasite growth was shown to be suppressed by a few of the inhibitors targeting Ddi1 present in these parasites. In this study, the binding of HIV protease inhibitors to the RVP domain of Ddi1 from and ; and the binding of ubiquitin to the ubiquitin-associated domain of Ddi1 from these two parasites were established using Biolayer Interferometry. The crystal structures of the RVP domains of Ddi1 from and were determined; they form homodimers similar to those observed in HIV protease and the reported structures of the same domain from , and humans. The native form of the domain showed an open dimeric structure and a normal mode analysis revealed that it can take up a closed conformation resulting from relative movements of the subunits. Based on the crystal structure of the RVP domain of Ddi1 from , a seven residue peptide inhibitor was designed and it was shown to bind to the RVP domain of Ddi1 from by Biolayer Interferometry. This peptide was modified using computational methods and was shown to have a better affinity than the initial peptide.

PubMed: 35677776
DOI: 10.1016/j.crstbi.2022.05.003

Experimental method

X-RAY DIFFRACTION (2.8 Å)