7EF2

Crystal structure of maize SHH2 SAWADEE domain in complex with an H3K9me3 peptide

7EF2 の概要

• エントリーDOI: 10.2210/pdb7ef2/pdb
• 分子名称: HB transcription factor, Histone H3.2, ZINC ION, ... (4 entities in total)
• 機能のキーワード: sawadee domain, maize, shh2, gene regulation
• 由来する生物種: Zea mays (Maize); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 38518.10

構造登録者

Wang, Y.,Du, J. (登録日: 2021-03-20, 公開日: 2021-06-30, 最終更新日: 2023-11-29)

主引用文献

Wang, Y.,Zhou, X.,Luo, J.,Lv, S.,Liu, R.,Du, X.,Jia, B.,Yuan, F.,Zhang, H.,Du, J.
Recognition of H3K9me1 by maize RNA-directed DNA methylation factor SHH2.
J Integr Plant Biol, 63:1091-1096, 2021

PubMed Abstract: RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has extensive cross-talk with histone modifications. Here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural studies reveal that H3K9me1 recognition is achieved by recognition of the methyl group via a classic aromatic cage and hydrogen-bonding and salt-bridge interactions with the free protons of the mono-methyllysine. The di- and tri-methylation states disrupt the polar interactions, decreasing the binding affinity. Our study reveals a mono-methyllysine recognition mechanism which potentially links RdDM to H3K9me1 in maize.

PubMed: 33913587
DOI: 10.1111/jipb.13103

実験手法

X-RAY DIFFRACTION (2 Å)