7EEA

Cyanophage Pam1 tailspike receptor-binding domain

Summary for 7EEA

• Entry DOI: 10.2210/pdb7eea/pdb
• Descriptor: Short-tailed cyanophage tailspike receptor-binding domain (2 entities in total)
• Functional Keywords: tailspike receptor-binding domain, viral protein
• Biological source: unidentified
• Total number of polymer chains: 3
• Total formula weight: 211153.29

Authors

Zhang, J.T.,Jiang, Y.L.,Zhou, C.Z. (deposition date: 2021-03-18, release date: 2021-10-20, Last modification date: 2024-05-29)

Primary citation

Zhang, J.T.,Yang, F.,Du, K.,Li, W.F.,Chen, Y.,Jiang, Y.L.,Li, Q.,Zhou, C.Z.
Structure and assembly pattern of a freshwater short-tailed cyanophage Pam1.
Structure, 30:240-251.e4, 2022

PubMed Abstract: Despite previous structural analyses of bacteriophages, quite little is known about the structures and assembly patterns of cyanophages. Using cryo-EM combined with crystallography, we solve the near-atomic-resolution structure of a freshwater short-tailed cyanophage, Pam1, which comprises a 400-Å-long tail and an icosahedral capsid of 650 Å in diameter. The outer capsid surface is reinforced by trimeric cement proteins with a β-sandwich fold, which structurally resemble the distal motif of Pam1's tailspike, suggesting its potential role in host recognition. At the portal vertex, the dodecameric portal and connected adaptor, followed by a hexameric needle head, form a DNA ejection channel, which is sealed by a trimeric needle. Moreover, we identify a right-handed rifling pattern that might help DNA to revolve along the wall of the ejection channel. Our study reveals the precise assembly pattern of a cyanophage and lays the foundation to support its practical biotechnological and environmental applications.

PubMed: 34727518
DOI: 10.1016/j.str.2021.10.004

Experimental method

X-RAY DIFFRACTION (2.671 Å)