7EDA

Structure of monomeric photosystem II

7EDA の概要

• エントリーDOI: 10.2210/pdb7eda/pdb
• EMDBエントリー: 31062
• 分子名称: Photosystem II protein D1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (36 entities in total)
• 機能のキーワード: photosynthesis, photosystem, membrane protein
• 由来する生物種: Thermosynechococcus vulcanus; 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 353029.45

構造登録者

Yu, H.,Hamaguchi, T.,Nakajima, Y.,Kato, K.,kawakami, K.,Akita, F.,Yonekura, K.,Shen, J.R. (登録日: 2021-03-15, 公開日: 2021-07-07, 最終更新日: 2026-08-12)

主引用文献

Yu, H.,Hamaguchi, T.,Nakajima, Y.,Kato, K.,Kawakami, K.,Akita, F.,Yonekura, K.,Shen, J.R.
Cryo-EM structure of monomeric photosystem II at 2.78 angstrom resolution reveals factors important for the formation of dimer.
Biochim Biophys Acta Bioenerg, 1862:148471-148471, 2021

PubMed Abstract: Photosystem II (PSII) functions mainly as a dimer to catalyze the light energy conversion and water oxidation reactions. However, monomeric PSII also exists and functions in vivo in some cases. The crystal structure of monomeric PSII has been solved at 3.6 Å resolution, but it is still not clear which factors contribute to the formation of the dimer. Here, we solved the structure of PSII monomer at a resolution of 2.78 Å using cryo-electron microscopy (cryo-EM). From our cryo-EM density map, we observed apparent differences in pigments and lipids in the monomer-monomer interface between the PSII monomer and dimer. One β-carotene and two sulfoquinovosyl diacylglycerol (SQDG) molecules are found in the monomer-monomer interface of the dimer structure but not in the present monomer structure, although some SQDG and other lipid molecules are found in the analogous region of the low-resolution crystal structure of the monomer, or cryo-EM structure of an apo-PSII monomer lacking the extrinsic proteins from Synechocystis sp. PCC 6803. In the current monomer structure, a large part of the PsbO subunit was also found to be disordered. These results indicate the importance of the β-carotene, SQDG and PsbO in formation of the PSII dimer.

PubMed: 34216574
DOI: 10.1016/j.bbabio.2021.148471

実験手法

ELECTRON MICROSCOPY (2.78 Å)