7EB2

Cryo-EM structure of human GABA(B) receptor-Gi protein complex

7EB2 の概要

• エントリーDOI: 10.2210/pdb7eb2/pdb
• EMDBエントリー: 31049
• 分子名称: Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (8 entities in total)
• 機能のキーワード: gabab, cryo-em, gpcr, gi, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 314386.07

構造登録者

Shen, C.,Mao, C.,Xu, C.,Jin, N.,Zhang, H.,Shen, D.,Shen, Q.,Wang, X.,Hou, T.,Rondard, P.,Chen, Z.,Pin, J.,Zhang, Y.,Liu, J. (登録日: 2021-03-08, 公開日: 2021-05-05, 最終更新日: 2024-10-23)

主引用文献

Shen, C.,Mao, C.,Xu, C.,Jin, N.,Zhang, H.,Shen, D.D.,Shen, Q.,Wang, X.,Hou, T.,Chen, Z.,Rondard, P.,Pin, J.P.,Zhang, Y.,Liu, J.
Structural basis of GABA B receptor-G i protein coupling.
Nature, 594:594-598, 2021

PubMed Abstract: G-protein-coupled receptors (GPCRs) have central roles in intercellular communication. Structural studies have revealed how GPCRs can activate G proteins. However, whether this mechanism is conserved among all classes of GPCR remains unknown. Here we report the structure of the class-C heterodimeric GABA receptor, which is activated by the inhibitory transmitter GABA, in its active form complexed with G protein. We found that a single G protein interacts with the GB2 subunit of the GABA receptor at a site that mainly involves intracellular loop 2 on the side of the transmembrane domain. This is in contrast to the G protein binding in a central cavity, as has been observed with other classes of GPCR. This binding mode results from the active form of the transmembrane domain of this GABA receptor being different from that of other GPCRs, as it shows no outside movement of transmembrane helix 6. Our work also provides details of the inter- and intra-subunit changes that link agonist binding to G-protein activation in this heterodimeric complex.

PubMed: 33911284
DOI: 10.1038/s41586-021-03507-1

実験手法

ELECTRON MICROSCOPY (3.5 Å)