7EAP

Crystal structure of IpeA-XXXG complex

7EAP の概要

• エントリーDOI: 10.2210/pdb7eap/pdb
• 分子名称: Fn3_like domain-containing protein, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: glycoside hydrolase family 3, isoprimeverose-producing oligoxyloglucan hydrolase, xyloglucan oligosaccharides, hydrolase
• 由来する生物種: Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 87196.23

構造登録者

Matsuzawa, T.,Watanabe, M.,Nakamichi, Y.,Akita, H.,Yaoi, K. (登録日: 2021-03-08, 公開日: 2022-03-16, 最終更新日: 2024-11-06)

主引用文献

Matsuzawa, T.,Watanabe, M.,Nakamichi, Y.,Akita, H.,Yaoi, K.
Structural basis for the catalytic mechanism of the glycoside hydrolase family 3 isoprimeverose-producing oligoxyloglucan hydrolase from Aspergillus oryzae.
Febs Lett., 596:1944-1954, 2022

PubMed Abstract: Aspergillus oryzae isoprimeverose-producing oligoxyloglucan hydrolase (IpeA) releases isoprimeverose units (α-d-xylopyranosyl-(1→6)-d-glucose) from the non-reducing end of xyloglucan oligosaccharides and belongs to glycoside hydrolase family 3. In this paper, we report the X-ray crystal structure of the IpeA complexed with a xyloglucan oligosaccharide, (XXXG: Glc Xyl ). Trp515 of IpeA plays a critical role in XXXG recognition at positive subsites. In addition, docking simulation of IpeA-XXXG suggested that two Tyr residues (Tyr268 and Tyr445) are involved in the catalytic reaction mechanism of IpeA. Tyr268 plays an important role in product turnover, whereas Tyr445 stabilizes the acid/base Glu524 residue, which serves as a proton donor. Our findings indicate that the substrate recognition machinery of IpeA is specifically adapted to xyloglucan oligosaccharides.

PubMed: 35717558
DOI: 10.1002/1873-3468.14427

実験手法

X-RAY DIFFRACTION (1.42 Å)