7E9Y
Crystal structure of eLACCO1
Summary for 7E9Y
| Entry DOI | 10.2210/pdb7e9y/pdb |
| Descriptor | Lactate-binding periplasmic protein TTHA0766,Lactate-binding periplasmic protein TTHA0766, CALCIUM ION, (2S)-2-HYDROXYPROPANOIC ACID, ... (4 entities in total) |
| Functional Keywords | biosensor, genetically encoded fluorescent biosensor, extracellular, l-lactate, fluorescent protein |
| Biological source | Thermus thermophilus HB8 More |
| Total number of polymer chains | 1 |
| Total formula weight | 70188.41 |
| Authors | Wen, Y.,Campbell, R.E.,Lemieux, M.J.,Nasu, Y. (deposition date: 2021-03-05, release date: 2021-12-22, Last modification date: 2023-11-29) |
| Primary citation | Nasu, Y.,Murphy-Royal, C.,Wen, Y.,Haidey, J.N.,Molina, R.S.,Aggarwal, A.,Zhang, S.,Kamijo, Y.,Paquet, M.E.,Podgorski, K.,Drobizhev, M.,Bains, J.S.,Lemieux, M.J.,Gordon, G.R.,Campbell, R.E. A genetically encoded fluorescent biosensor for extracellular L-lactate. Nat Commun, 12:7058-7058, 2021 Cited by PubMed Abstract: L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue. PubMed: 34873165DOI: 10.1038/s41467-021-27332-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.25 Å) |
Structure validation
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