7E9Y

Crystal structure of eLACCO1

7E9Y の概要

• エントリーDOI: 10.2210/pdb7e9y/pdb
• 分子名称: Lactate-binding periplasmic protein TTHA0766,Lactate-binding periplasmic protein TTHA0766, CALCIUM ION, (2S)-2-HYDROXYPROPANOIC ACID, ... (4 entities in total)
• 機能のキーワード: biosensor, genetically encoded fluorescent biosensor, extracellular, l-lactate, fluorescent protein
• 由来する生物種: Thermus thermophilus HB8; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 70188.41

構造登録者

Wen, Y.,Campbell, R.E.,Lemieux, M.J.,Nasu, Y. (登録日: 2021-03-05, 公開日: 2021-12-22, 最終更新日: 2026-03-18)

主引用文献

Nasu, Y.,Murphy-Royal, C.,Wen, Y.,Haidey, J.N.,Molina, R.S.,Aggarwal, A.,Zhang, S.,Kamijo, Y.,Paquet, M.E.,Podgorski, K.,Drobizhev, M.,Bains, J.S.,Lemieux, M.J.,Gordon, G.R.,Campbell, R.E.
A genetically encoded fluorescent biosensor for extracellular L-lactate.
Nat Commun, 12:7058-7058, 2021

PubMed Abstract: L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.

PubMed: 34873165
DOI: 10.1038/s41467-021-27332-2

実験手法

X-RAY DIFFRACTION (2.25 Å)