7E62

Mouse TAB2 NZF in complex with Lys6-linked diubiquitin

Summary for 7E62

• Entry DOI: 10.2210/pdb7e62/pdb
• Descriptor: ubiquitin, Ubiquitin, TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, ... (6 entities in total)
• Functional Keywords: ubiquitin, protein binding
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 6
• Total formula weight: 47352.58

Authors

Sato, Y.,Li, Y.,Okatsu, K.,Fukai, S. (deposition date: 2021-02-21, release date: 2021-08-18, Last modification date: 2023-11-29)

Primary citation

Li, Y.,Okatsu, K.,Fukai, S.,Sato, Y.
Structural basis for specific recognition of K6-linked polyubiquitin chains by the TAB2 NZF domain.
Biophys.J., 120:3355-3362, 2021

PubMed Abstract: TAK1-binding protein 2 (TAB2) has generally been considered to bind specifically to K63-linked polyubiquitin chains via its C-terminal Npl4 zinc-finger (NZF) domain. However, a recent study showed that the NZF domain of TAB2 (TAB2-NZF) could also interact with K6-linked polyubiquitin chains. Here, we report the crystal structure of TAB2-NZF in complex with K6-linked diubiquitin (K6-Ub) at 1.99-Å resolution. TAB2-NZF simultaneously interacts with the distal and proximal ubiquitin moieties of K6-Ub. By comparing the structures of TAB2-NZF in complex with K6-Ub and with K63-linked diubiquitin (K63-Ub), we reveal that the binding mechanism of TAB2-NZF with K6-Ub is similar to that with K63-Ub, except for the flexible C-terminal region of the distal ubiquitin. Therefore, we conclude that the C-terminal flexibility of the distal ubiquitin contributes to the dual specificity of TAB2-NZF toward K6- and K63-linked ubiquitin chains. This study provides important insights into the functions of K6-linked ubiquitin chains, which are currently unclear.

PubMed: 34242591
DOI: 10.1016/j.bpj.2021.06.037

Experimental method

X-RAY DIFFRACTION (1.99 Å)