7E53

Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution

7E53 の概要

• エントリーDOI: 10.2210/pdb7e53/pdb
• 分子名称: Green fluorescent protein, Green fluorescent protein's nanobody nb2 (3 entities in total)
• 機能のキーワード: aequorea victoria, camelus bactrianus, fluorescent protein
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40082.65

構造登録者

Ding, Y.,Zhong, P.Y. (登録日: 2021-02-16, 公開日: 2021-10-13, 最終更新日: 2024-11-13)

主引用文献

Zhong, P.,Wang, Z.,Cheng, S.,Zhang, Y.,Jiang, H.,Liu, R.,Ding, Y.
Structural insights into two distinct nanobodies recognizing the same epitope of green fluorescent protein.
Biochem.Biophys.Res.Commun., 565:57-63, 2021

PubMed Abstract: Green fluorescent protein (GFP) and its derivatives are widely used in biomedical research, and the manipulation of GFP-tagged proteins by GFP-specific binders is highly desired. However, structural information on how these binders bind with GFP is still lacking. In this study, we determined the crystal structure of the nanobody Nb2 complexed with superfolder GFP (sfGFP) at a resolution of 2.2 Å. Interestingly, although the complementarity-determining regions (CDRs) of Nb2 and LaG16 sequences were only 29.7% identical, they both bound to the same epitope of GFP and existed in the same orientation. Structural analysis indicated that they achieved similar binding characteristics through different mechanisms. We further verified the kinetics and thermodynamics of binding by biolayer interferometry (BLI) and isothermal titration calorimetry (ITC). Nb2 showed a slightly higher binding affinity for sfGFP than LaG16. The stability of GFP-specific nanobodies was verified by nano differential scanning fluorimetry (nanoDSF). Nb2 exhibited the highest melting temperature (Tm); thus, Nb2 is a promising GFP nanobody candidate for use in applications requiring harsh testing conditions. We also compared the binding sites of available GFP nanobodies and showed that some of them can simultaneously bind with GFP and assemble into multifunctional complexes to manipulate GFP-tagged target proteins. Our results provide atomic-scale binding information for Nb2-sfGFP, which is important for the further development of GFP-nanobody based fusion protein manipulation techniques.

PubMed: 34098312
DOI: 10.1016/j.bbrc.2021.05.089

実験手法

X-RAY DIFFRACTION (2.21 Å)