7E3F

Crystal structure of Trypanosoma brucei cathepsin B Y217C/S275C mutant

7E3F の概要

• エントリーDOI: 10.2210/pdb7e3f/pdb
• 分子名称: Cysteine peptidase C (CPC), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: in cell crystal, hydrolase
• 由来する生物種: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35393.22

構造登録者

Abe, S.,Pham, T.T.,Negishi, H.,Yamashita, K.,Hirata, K.,Ueno, T. (登録日: 2021-02-08, 公開日: 2021-12-15, 最終更新日: 2024-11-13)

主引用文献

Abe, S.,Pham, T.T.,Negishi, H.,Yamashita, K.,Hirata, K.,Ueno, T.
Design of an In-Cell Protein Crystal for the Environmentally Responsive Construction of a Supramolecular Filament.
Angew.Chem.Int.Ed.Engl., 60:12341-12345, 2021

PubMed Abstract: Protein assemblies can be designed for development of nano-bio materials. This has been achieved by modulating protein-protein interactions. However, fabrication of highly ordered protein assemblies remains challenging. Protein crystals, which have highly ordered arrangements of protein molecules, provide useful source matrices for synthesizing artificial protein assemblies. Here, we describe construction of a supramolecular filament structure by engineering covalent and non-covalent interactions in a protein crystal. Performing in-cell crystallization of Trypanosoma brucei cysteine protease cathepsin B (TbCatB), we achieved a precise arrangement of protein molecules while suppressing random aggregation due to disulfide bonds. We succeeded in synthesizing bundled filament from the crystals by autoxidation of cysteinyl thiols after the isolation of the crystals from living cells.

PubMed: 33759310
DOI: 10.1002/anie.202102039

実験手法

X-RAY DIFFRACTION (2.35 Å)