7E34

Crystal structure of SUN1-Speedy A-CDK2

7E34 の概要

• エントリーDOI: 10.2210/pdb7e34/pdb
• 分子名称: Cyclin-dependent kinase 2, Speedy protein A, SUN domain-containing protein 1, ... (6 entities in total)
• 機能のキーワード: telomere, meiosis, kinase, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57726.71

構造登録者

Chen, Y.,Huang, C.,Wu, J.,Lei, M. (登録日: 2021-02-08, 公開日: 2021-04-14, 最終更新日: 2023-11-29)

主引用文献

Chen, Y.,Wang, Y.,Chen, J.,Zuo, W.,Fan, Y.,Huang, S.,Liu, Y.,Chen, G.,Li, Q.,Li, J.,Wu, J.,Bian, Q.,Huang, C.,Lei, M.
The SUN1-SPDYA interaction plays an essential role in meiosis prophase I.
Nat Commun, 12:3176-3176, 2021

PubMed Abstract: Chromosomes pair and synapse with their homologous partners to segregate correctly at the first meiotic division. Association of telomeres with the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex composed of SUN1 and KASH5 enables telomere-led chromosome movements and telomere bouquet formation, facilitating precise pairwise alignment of homologs. Here, we identify a direct interaction between SUN1 and Speedy A (SPDYA) and determine the crystal structure of human SUN1-SPDYA-CDK2 ternary complex. Analysis of meiosis prophase I process in SPDYA-binding-deficient SUN1 mutant mice reveals that the SUN1-SPDYA interaction is required for the telomere-LINC complex connection and the assembly of a ring-shaped telomere supramolecular architecture at the nuclear envelope, which is critical for efficient homologous pairing and synapsis. Overall, our results provide structural insights into meiotic telomere structure that is essential for meiotic prophase I progression.

PubMed: 34039995
DOI: 10.1038/s41467-021-23550-w

実験手法

X-RAY DIFFRACTION (3.19 Å)