7E2K

Crystal structure of the RWD domain of human GCN2 - 1

7E2K の概要

• エントリーDOI: 10.2210/pdb7e2k/pdb
• 分子名称: eIF-2-alpha kinase GCN2 (2 entities in total)
• 機能のキーワード: activator, kinase, rna-binding, serine/threonine-protein kinase, transferase, trna-binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15301.24

構造登録者

Hei, Z.,Zhou, J.,Liu, Z.,Wang, J.,Fang, P. (登録日: 2021-02-05, 公開日: 2021-03-17, 最終更新日: 2023-11-29)

主引用文献

Hei, Z.,Wu, S.,Zheng, L.,Zhou, J.,Liu, Z.,Wang, J.,Fang, P.
Crystal structures reveal a novel dimer of the RWD domain of human general control nonderepressible 2.
Biochem.Biophys.Res.Commun., 549:164-170, 2021

PubMed Abstract: General control nonderepressible 2 (GCN2) is a serine/threonine protein kinase, detecting a variety of stresses including amino acid starvation, reactive oxygen species, etc. in eukaryotic cells. Activation of GCN2 requires the interaction of the N-terminal RWD domain with the upstream GCN1 protein and the dimerization by the kinase domain. In this study, we determined two crystal structures of the RWD domain of human GCN2 in two different crystal packing modes. These two different crystal structures reveal a same dimer of the RWD domain, which has not been reported in previous studies. We further confirmed this novel dimer interaction in solution using gel filtration experiments, and in human embryonic kidney (HEK) 293 cells using bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (co-IP) assays. Together, this study discovers a potential protein-protein interface on the RWD domain of human GCN2, and suggests a possible regulation between the interaction of GCN1 and the formation of GCN2 dimer.

PubMed: 33676185
DOI: 10.1016/j.bbrc.2021.02.111

実験手法

X-RAY DIFFRACTION (2.041 Å)