7DXA

PSII intermediate Psb28-RC47

7DXA の概要

• エントリーDOI: 10.2210/pdb7dxa/pdb
• EMDBエントリー: 30902
• 分子名称: Photosystem II reaction center Psb28 protein, Photosystem II reaction center protein L, Photosystem II reaction center protein M, ... (27 entities in total)
• 機能のキーワード: photosystem ii; cryo-em; assembly; repair; psb28; tsl0063, plant protein
• 由来する生物種: Thermosynechococcus vulcanus; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 232923.20

構造登録者

Sui, S.F.,Shen, J.R.,Han, G.Y.,Xiao, Y.N.,Huang, G.Q. (登録日: 2021-01-18, 公開日: 2021-06-30, 最終更新日: 2025-07-02)

主引用文献

Xiao, Y.,Huang, G.,You, X.,Zhu, Q.,Wang, W.,Kuang, T.,Han, G.,Sui, S.F.,Shen, J.R.
Structural insights into cyanobacterial photosystem II intermediates associated with Psb28 and Tsl0063.
Nat.Plants, 7:1132-1142, 2021

PubMed Abstract: Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyses light-induced water oxidation, leading to the conversion of light energy into chemical energy and the release of dioxygen. We analysed the structures of two Psb28-bound PSII intermediates, Psb28-RC47 and Psb28-PSII, purified from a psbV-deletion strain of the thermophilic cyanobacterium Thermosynechococcus vulcanus, using cryo-electron microscopy. Both Psb28-RC47 and Psb28-PSII bind one Psb28, one Tsl0063 and an unknown subunit. Psb28 is located at the cytoplasmic surface of PSII and interacts with D1, D2 and CP47, whereas Tsl0063 is a transmembrane subunit and binds at the side of CP47/PsbH. Substantial structural perturbations are observed at the acceptor side, which result in conformational changes of the quinone (Q) and non-haem iron binding sites and thus may protect PSII from photodamage during assembly. These results provide a solid structural basis for understanding the assembly process of native PSII.

PubMed: 34226692
DOI: 10.1038/s41477-021-00961-7

実験手法

ELECTRON MICROSCOPY (3.14 Å)