7DVE

Crystal structure of FAD-dependent C-glycoside oxidase

7DVE の概要

• エントリーDOI: 10.2210/pdb7dve/pdb
• 分子名称: 6'''-hydroxyparomomycin C oxidase, FLAVIN-ADENINE DINUCLEOTIDE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: fad-dependent c-glycoside oxidase, oxidoreductase
• 由来する生物種: Microbacterium trichothecenolyticum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113546.90

構造登録者

Senda, M.,Watanabe, S.,Kumano, T.,Kobayashi, M.,Senda, T. (登録日: 2021-01-13, 公開日: 2021-09-08, 最終更新日: 2023-11-29)

主引用文献

Kumano, T.,Hori, S.,Watanabe, S.,Terashita, Y.,Yu, H.Y.,Hashimoto, Y.,Senda, T.,Senda, M.,Kobayashi, M.
FAD-dependent C -glycoside-metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: -glycosides have a unique structure, in which an anomeric carbon of a sugar is directly bonded to the carbon of an aglycone skeleton. One of the natural -glycosides, carminic acid, is utilized by the food, cosmetic, and pharmaceutical industries, for a total of more than 200 tons/y worldwide. However, a metabolic pathway of carminic acid has never been identified. In this study, we isolated the previously unknown carminic acid-catabolizing microorganism and discovered a flavoenzyme "-glycoside 3-oxidase" named CarA that catalyzes oxidation of the sugar moiety of carminic acid. A Basic Local Alignment Search Tool (BLAST) search demonstrated that CarA homologs were distributed in soil microorganisms but not intestinal ones. In addition to CarA, two CarA homologs were cloned and heterologously expressed, and their biochemical properties were determined. Furthermore, a crystal structure of one homolog was determined. Together with the biochemical analysis, the crystal structure and a mutagenesis analysis of CarA revealed the mechanisms underlying their substrate specificity and catalytic reaction. Our study suggests that CarA and its homologs play a crucial role in the metabolism of -glycosides in nature.

PubMed: 34583991
DOI: 10.1073/pnas.2106580118

実験手法

X-RAY DIFFRACTION (2.4 Å)