7DRV

Structural basis of SARS-CoV-2-closely-related bat coronavirus RaTG13 to hACE2

7DRV の概要

• エントリーDOI: 10.2210/pdb7drv/pdb
• 分子名称: Angiotensin-converting enzyme 2, Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: ratg13, rbd, sars-cov-2, ace2, viral protein, hydrolase-viral protein complex, hydrolase/viral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 190888.08

構造登録者

Liu, K.F.,Pan, X.Q.,Li, L.J.,Feng, Y.,Meng, Y.M.,Zhang, Y.F.,Wu, L.L.,Chen, Q.,Zheng, A.Q.,Song, C.L.,Jia, Y.F.,Niu, S.,Qiao, C.P.,Zhao, X.,Ma, D.L.,Ma, X.P.,Tan, S.G.,Qi, J.X.,Gao, G.F.,Wang, Q.H. (登録日: 2020-12-29, 公開日: 2021-08-25, 最終更新日: 2024-11-13)

主引用文献

Liu, K.,Pan, X.,Li, L.,Yu, F.,Zheng, A.,Du, P.,Han, P.,Meng, Y.,Zhang, Y.,Wu, L.,Chen, Q.,Song, C.,Jia, Y.,Niu, S.,Lu, D.,Qiao, C.,Chen, Z.,Ma, D.,Ma, X.,Tan, S.,Zhao, X.,Qi, J.,Gao, G.F.,Wang, Q.
Binding and molecular basis of the bat coronavirus RaTG13 virus to ACE2 in humans and other species.
Cell, 184:3438-3451.e10, 2021

PubMed Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide, causing a global pandemic. Bat-origin RaTG13 is currently the most phylogenetically related virus. Here we obtained the complex structure of the RaTG13 receptor binding domain (RBD) with human ACE2 (hACE2) and evaluated binding of RaTG13 RBD to 24 additional ACE2 orthologs. By substituting residues in the RaTG13 RBD with their counterparts in the SARS-CoV-2 RBD, we found that residue 501, the major position found in variants of concern (VOCs) 501Y.V1/V2/V3, plays a key role in determining the potential host range of RaTG13. We also found that SARS-CoV-2 could induce strong cross-reactive antibodies to RaTG13 and identified a SARS-CoV-2 monoclonal antibody (mAb), CB6, that could cross-neutralize RaTG13 pseudovirus. These results elucidate the receptor binding and host adaption mechanisms of RaTG13 and emphasize the importance of continuous surveillance of coronaviruses (CoVs) carried by animal reservoirs to prevent another spillover of CoVs.

PubMed: 34139177
DOI: 10.1016/j.cell.2021.05.031

実験手法

X-RAY DIFFRACTION (3.09 Å)