7DQK

A nicotine MATE transporter, Nicotiana tabacum MATE2 (NtMATE2)

7DQK の概要

• エントリーDOI: 10.2210/pdb7dqk/pdb
• 分子名称: Protein DETOXIFICATION, DI(HYDROXYETHYL)ETHER, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (6 entities in total)
• 機能のキーワード: multidrug efflux transporter, transport protein
• 由来する生物種: Nicotiana tabacum (Common tobacco)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111846.09

構造登録者

Tanaka, Y.,Tsukazaki, T.,Sasaki, A.,Iwaki, S. (登録日: 2020-12-24, 公開日: 2021-06-09, 最終更新日: 2023-11-29)

主引用文献

Tanaka, Y.,Iwaki, S.,Sasaki, A.,Tsukazaki, T.
Crystal structures of a nicotine MATE transporter provide insight into its mechanism of substrate transport.
Febs Lett., 595:1902-1913, 2021

PubMed Abstract: A transporter of the multidrug and toxic compound extrusion (MATE) family, Nicotiana tabacum MATE2 (NtMATE2), is located in the vacuole membrane of the tobacco plant root and is involved in the transportation of nicotine, a secondary or specialized metabolic compound in Solanaceae. Here, we report the crystal structures of NtMATE2 in its outward-facing forms. The overall structure has a bilobate V-shape with pseudo-symmetrical assembly of the N- and C-lobes. In one crystal structure, the C-lobe cavity of NtMATE2 interacts with an unidentified molecule that may partially mimic a substrate. In addition, NtMATE2-specific conformational transitions imply that an unprecedented movement of the transmembrane α-helix 7 is related to the release of the substrate into the vacuolar lumen.

PubMed: 34050946
DOI: 10.1002/1873-3468.14136

実験手法

X-RAY DIFFRACTION (3.5 Å)