7DP3

Human MCM8 N-terminal domain

7DP3 の概要

• エントリーDOI: 10.2210/pdb7dp3/pdb
• 分子名称: DNA helicase MCM8, ZINC ION (3 entities in total)
• 機能のキーワード: zinc finger, dna binding, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74459.29

構造登録者

Li, J.,Liu, L.,Liu, Y. (登録日: 2020-12-17, 公開日: 2021-05-19, 最終更新日: 2024-10-16)

主引用文献

Li, J.,Yu, D.,Liu, L.,Liang, H.,Ouyang, Q.,Liu, Y.
Structural study of the N-terminal domain of human MCM8/9 complex.
Structure, 29:1171-1181.e4, 2021

PubMed Abstract: MCM8/9 is a complex involved in homologous recombination (HR) repair pathway. MCM8/9 dysfunction can cause genome instability and result in primary ovarian insufficiency (POI). However, the mechanism underlying these effects is largely unknown. Here, we report crystal structures of the N-terminal domains (NTDs) of MCM8 and MCM9, and build a ring-shaped NTD structure based on a 6.6 Å resolution cryoelectron microscopy map. This shows that the MCM8/9 complex forms a 3:3 heterohexamer in an alternating pattern. A positively charged DNA binding channel and a putative ssDNA exit pathway for fork DNA unwinding are revealed. Based on the atomic model, the potential effects of the clinical POI mutants are interpreted. Surprisingly, the zinc-finger motifs are found to be capable of binding an iron atom as well. Overall, our results provide a model for the formation of the MCM8/9 complex and provide a path for further studies.

PubMed: 34043945
DOI: 10.1016/j.str.2021.05.006

実験手法

X-RAY DIFFRACTION (2.55 Å)