7DOG

Crystal structure of a nuclease and capping domain of SbcD from Staphylococcus aureus

7DOG の概要

• エントリーDOI: 10.2210/pdb7dog/pdb
• 分子名称: Nuclease SbcCD subunit D, MANGANESE (II) ION (2 entities in total)
• 機能のキーワード: dna nuclease, endonuclease, exonuclease, dna repair, hydrolase
• 由来する生物種: Staphylococcus aureus subsp. aureus Mu50
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73859.36

構造登録者

Lee, J.,Ha, N.-C. (登録日: 2020-12-14, 公開日: 2021-05-05, 最終更新日: 2023-11-29)

主引用文献

Lee, J.,Jo, I.,Ahn, J.,Hong, S.,Jeong, S.,Kwon, A.,Ha, N.C.
Crystal structure of the nuclease and capping domain of SbcD from Staphylococcus aureus.
J.Microbiol, 59:584-589, 2021

PubMed Abstract: The SbcCD complex is an essential component of the DNA double-strand break (DSB) repair system in bacteria. The bacterial SbcCD complex recognizes and cleaves the DNA ends in DSBs by ATP-dependent endo- and exonuclease activities as an early step of the DNA repair process. SbcD consists of nuclease, capping, and helix-loop-helix domains. Here, we present the crystal structure of a SbcD fragment from Staphylococcus aureus, which contained nuclease and capping domains, at a resolution of 2.9 Å. This structure shows a dimeric assembly similar to that of the corresponding domains of SbcD from Escherichia coli. The S. aureus SbcD fragment exhibited endonuclease activities on supercoiled DNA and exonuclease activity on linear and nicked DNA. This study contributes to the understanding of the molecular basis for how bacteria can resist sterilizing treatment, causing DNA damage.

PubMed: 33877576
DOI: 10.1007/s12275-021-1012-0

実験手法

X-RAY DIFFRACTION (2.91 Å)