7DL0

The mutant E310G/A314Y of 3,5-DAHDHcca complex with NADPH

7DL0 の概要

• エントリーDOI: 10.2210/pdb7dl0/pdb
• 分子名称: 3,5-diaminohexanoate dehydrogenase, DI(HYDROXYETHYL)ETHER, 1,2-ETHANEDIOL, ... (9 entities in total)
• 機能のキーワード: dehydrogenase, complex, nadph, oxidoreductase
• 由来する生物種: Cloacimonas acidaminovorans (strain Evry)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80187.42

構造登録者

Liu, N.,Wu, L.,Zhu, D.M.,Zhou, J.H. (登録日: 2020-11-25, 公開日: 2021-09-29, 最終更新日: 2024-05-29)

主引用文献

Liu, N.,Wu, L.,Feng, J.,Sheng, X.,Li, J.,Chen, X.,Li, J.,Liu, W.,Zhou, J.,Wu, Q.,Zhu, D.
Crystal Structures and Catalytic Mechanism of l-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of beta-Amino Acids.
Angew.Chem.Int.Ed.Engl., 60:10203-10210, 2021

PubMed Abstract: Amino acid dehydrogenases (AADHs) have shown considerable potential as biocatalysts in the asymmetric synthesis of chiral amino acids. However, compared to the widely studied α-AADHs, limited knowledge is available about β-AADHs that enable the synthesis of β-amino acids. Herein, we report the crystal structures of a l-erythro-3,5-diaminohexanoate dehydrogenase and its variants, the only known member of β-AADH family. Crystal structure analysis, site-directed mutagenesis studies and quantum chemical calculations revealed the differences in the substrate binding and catalytic mechanism from α-AADHs. A number of rationally engineered variants were then obtained with improved activity (by 110-800 times) toward various aliphatic β-amino acids without an enantioselectivity trade-off. Two β-amino acids were prepared by using the outstanding variants with excellent enantioselectivity (>99 % ee) and high isolated yields (86-87 %). These results provide important insights into the molecular mechanism of 3,5-DAHDH, and establish a solid foundation for further design of β-AADHs for the asymmetric synthesis of β-amino acids.

PubMed: 33624917
DOI: 10.1002/anie.202017225

実験手法

X-RAY DIFFRACTION (2.17 Å)