7DHP

Crystal structure of MazF from Deinococcus radiodurans

Summary for 7DHP

• Entry DOI: 10.2210/pdb7dhp/pdb
• Descriptor: Endoribonuclease MazF, SULFATE ION (3 entities in total)
• Functional Keywords: toxin-antitoxin, ribonuclease, toxin
• Biological source: Deinococcus radiodurans
• Total number of polymer chains: 2
• Total formula weight: 29561.36

Authors

Zhao, Y.,Dai, J. (deposition date: 2020-11-17, release date: 2021-03-17, Last modification date: 2023-11-29)

Primary citation

Dai, J.,Chen, Z.,Hou, J.,Wang, Y.,Guo, M.,Cao, J.,Wang, L.,Xu, H.,Tian, B.,Zhao, Y.
MazEF Toxin-Antitoxin System-Mediated DNA Damage Stress Response in Deinococcus radiodurans.
Front Genet, 12:632423-632423, 2021

PubMed Abstract: shows marked resistance to various types of DNA-damaging agents, including mitomycin C (MMC). A type II toxin-antitoxin (TA) system that responds to DNA damage stress was identified in , comprising the toxin MazF-dr and the antitoxin MazE-dr. The cleavage specificity of MazF-dr, an endoribonuclease, was previously characterized. Here, we further investigated the regulatory role of the MazEF system in the response to DNA damage stress in . The crystal structure of MazF (MazF-dr) was determined at a resolution of 1.3 Å and is the first structure of the toxin of the TA system of . MazF-dr forms a dimer mediated by the presence of interlocked loops. Transcriptional analysis revealed 650 downregulated genes in the wild-type (WT) strain, but not in the mutant strain, which are potentially regulated by MazEF-dr in response to MMC treatment. Some of these genes are involved in membrane trafficking and metal ion transportation. Subsequently, compared with the WT strain, the mutant strain exhibited much lower MMC-induced intracellular iron concentrations, reactive oxygen species (ROS), and protein carbonylation levels. These results provide evidence that MazEF-mediated cell death in might be caused by an increase in ROS accumulation upon DNA damage stress.

PubMed: 33679894
DOI: 10.3389/fgene.2021.632423

Experimental method

X-RAY DIFFRACTION (1.3 Å)