7DCS

Crystal structure of HSF1 DNA-binding domain in complex with 3-site HSE DNA (23 bp)

Summary for 7DCS

• Entry DOI: 10.2210/pdb7dcs/pdb
• Descriptor: Heat shock factor protein 1, DNA (5'-D(*TP*GP*GP*CP*GP*TP*TP*CP*TP*AP*GP*AP*AP*TP*AP*TP*TP*CP*GP*CP*GP*GP*A)-3'), DNA (5'-D(*AP*TP*CP*CP*GP*CP*GP*AP*AP*TP*AP*TP*TP*CP*TP*AP*GP*AP*AP*CP*GP*CP*C)-3'), ... (5 entities in total)
• Functional Keywords: dna binding protein-dna complex, dna binding protein/dna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 10
• Total formula weight: 107641.39

Authors

Feng, N.,Liu, W. (deposition date: 2020-10-27, release date: 2021-07-14, Last modification date: 2023-11-29)

Primary citation

Feng, N.,Feng, H.,Wang, S.,Punekar, A.S.,Ladenstein, R.,Wang, D.C.,Zhang, Q.,Ding, J.,Liu, W.
Structures of heat shock factor trimers bound to DNA.
Iscience, 24:102951-102951, 2021

PubMed Abstract: Heat shock factor 1 (HSF1) and 2 (HSF2) play distinct but overlapping regulatory roles in maintaining cellular proteostasis or mediating cell differentiation and development. Upon activation, both HSFs trimerize and bind to heat shock elements (HSEs) present in the promoter region of target genes. Despite structural insights gained from recent studies, structures reflecting the physiological architecture of this transcriptional machinery remains to be determined. Here, we present co-crystal structures of human HSF1 and HSF2 trimers bound to DNA, which reveal a triangular arrangement of the three DNA-binding domains (DBDs) with protein-protein interactions largely mediated by the wing domain. Two structural properties, different flexibility of the wing domain and local DNA conformational changes induced by HSF binding, seem likely to contribute to the subtle differential specificity between HSF1 and HSF2. Besides, two more structures showing DBDs bound to "two-site" head-to-head HSEs were determined as additions to the published tail-to-tail dimer-binding structures.

PubMed: 34458700
DOI: 10.1016/j.isci.2021.102951

Experimental method

X-RAY DIFFRACTION (2.4 Å)