7D6E

Structural insights into membrane remodeling by SNX1

7D6E の概要

• エントリーDOI: 10.2210/pdb7d6e/pdb
• EMDBエントリー: 30593
• 分子名称: Sorting nexin-1 (1 entity in total)
• 機能のキーワード: coat complex, membrane deformation, lipid binding protein, helical assembly, protein transport
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 1075335.97

構造登録者

Zhang, Y.,Pang, X.,Sun, F. (登録日: 2020-09-30, 公開日: 2021-03-17, 最終更新日: 2024-03-27)

主引用文献

Zhang, Y.,Pang, X.,Li, J.,Xu, J.,Hsu, V.W.,Sun, F.
Structural insights into membrane remodeling by SNX1.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: The sorting nexin (SNX) family of proteins deform the membrane to generate transport carriers in endosomal pathways. Here, we elucidate how a prototypic member, SNX1, acts in this process. Performing cryoelectron microscopy, we find that SNX1 assembles into a protein lattice that consists of helical rows of SNX1 dimers wrapped around tubular membranes in a crosslinked fashion. We also visualize the details of this structure, which provides a molecular understanding of how various parts of SNX1 contribute to its ability to deform the membrane. Moreover, we have compared the SNX1 structure with a previously elucidated structure of an endosomal coat complex formed by retromer coupled to a SNX, which reveals how the molecular organization of the SNX in this coat complex is affected by retromer. The comparison also suggests insight into intermediary stages of assembly that results in the formation of the retromer-SNX coat complex on the membrane.

PubMed: 33658379
DOI: 10.1073/pnas.2022614118

実験手法

ELECTRON MICROSCOPY (10 Å)