7D10

Human NKCC1

7D10 の概要

• エントリーDOI: 10.2210/pdb7d10/pdb
• EMDBエントリー: 30542
• 分子名称: Solute carrier family 12 member 2, PALMITIC ACID (2 entities in total)
• 機能のキーワード: membrane protein, transporter
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 264192.54

構造登録者

Zhang, S.,Yang, M. (登録日: 2020-09-12, 公開日: 2021-04-14, 最終更新日: 2024-05-29)

主引用文献

Zhang, S.,Zhou, J.,Zhang, Y.,Liu, T.,Friedel, P.,Zhuo, W.,Somasekharan, S.,Roy, K.,Zhang, L.,Liu, Y.,Meng, X.,Deng, H.,Zeng, W.,Li, G.,Forbush, B.,Yang, M.
The structural basis of function and regulation of neuronal cotransporters NKCC1 and KCC2.
Commun Biol, 4:226-226, 2021

PubMed Abstract: NKCC and KCC transporters mediate coupled transport of Na+K+Cl and K+Cl across the plasma membrane, thus regulating cell Cl concentration and cell volume and playing critical roles in transepithelial salt and water transport and in neuronal excitability. The function of these transporters has been intensively studied, but a mechanistic understanding has awaited structural studies of the transporters. Here, we present the cryo-electron microscopy (cryo-EM) structures of the two neuronal cation-chloride cotransporters human NKCC1 (SLC12A2) and mouse KCC2 (SLC12A5), along with computational analysis and functional characterization. These structures highlight essential residues in ion transport and allow us to propose mechanisms by which phosphorylation regulates transport activity.

PubMed: 33597714
DOI: 10.1038/s42003-021-01750-w

実験手法

ELECTRON MICROSCOPY (3.52 Å)