7D0R

Crystal structure of human HBO1-BRPF2 in complex with crotonoyl-coenzyme A

7D0R の概要

• エントリーDOI: 10.2210/pdb7d0r/pdb
• 分子名称: Histone acetyltransferase KAT7, BRD1 protein, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: histone acetyltransferase, complex, hbo1, brpf2, crotonoyl-coa, acylation, transferase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39259.38

構造登録者

Li, W.,Ding, J. (登録日: 2020-09-11, 公開日: 2021-07-07, 最終更新日: 2023-11-29)

主引用文献

Xiao, Y.,Li, W.,Yang, H.,Pan, L.,Zhang, L.,Lu, L.,Chen, J.,Wei, W.,Ye, J.,Li, J.,Li, G.,Zhang, Y.,Tan, M.,Ding, J.,Wong, J.
HBO1 is a versatile histone acyltransferase critical for promoter histone acylations.
Nucleic Acids Res., 49:8037-8059, 2021

PubMed Abstract: Recent studies demonstrate that histones are subjected to a series of short-chain fatty acid modifications that is known as histone acylations. However, the enzymes responsible for histone acylations in vivo are not well characterized. Here, we report that HBO1 is a versatile histone acyltransferase that catalyzes not only histone acetylation but also propionylation, butyrylation and crotonylation both in vivo and in vitro and does so in a JADE or BRPF family scaffold protein-dependent manner. We show that the minimal HBO1/BRPF2 complex can accommodate acetyl-CoA, propionyl-CoA, butyryl-CoA and crotonyl-CoA. Comparison of CBP and HBO1 reveals that they catalyze histone acylations at overlapping as well as distinct sites, with HBO1 being the key enzyme for H3K14 acylations. Genome-wide chromatin immunoprecipitation assay demonstrates that HBO1 is highly enriched at and contributes to bulk histone acylations on the transcriptional start sites of active transcribed genes. HBO1 promoter intensity highly correlates with the level of promoter histone acylation, but has no significant correlation with level of transcription. We also show that HBO1 is associated with a subset of DNA replication origins. Collectively our study establishes HBO1 as a versatile histone acyltransferase that links histone acylations to promoter acylations and selection of DNA replication origins.

PubMed: 34259319
DOI: 10.1093/nar/gkab607

実験手法

X-RAY DIFFRACTION (1.95 Å)