7CYP

Complex of SARS-CoV-2 spike trimer with its neutralizing antibody HB27

7CYP の概要

• エントリーDOI: 10.2210/pdb7cyp/pdb
• 関連するPDBエントリー: 7CYH
• EMDBエントリー: 30500 30503
• 分子名称: SARS-CoV-2 Spike glycoprotein, Light chain of HB27, Heavy chain of HB27, ... (5 entities in total)
• 機能のキーワード: sars-cov-2 spike, neutralizing antibody, complex, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 488893.47

構造登録者

Wang, X.,Zhu, L. (登録日: 2020-09-04, 公開日: 2021-06-09, 最終更新日: 2025-07-02)

主引用文献

Zhu, L.,Deng, Y.Q.,Zhang, R.R.,Cui, Z.,Sun, C.Y.,Fan, C.F.,Xing, X.,Huang, W.,Chen, Q.,Zhang, N.N.,Ye, Q.,Cao, T.S.,Wang, N.,Wang, L.,Cao, L.,Wang, H.,Kong, D.,Ma, J.,Luo, C.,Zhang, Y.,Nie, J.,Sun, Y.,Lv, Z.,Shaw, N.,Li, Q.,Li, X.F.,Hu, J.,Xie, L.,Rao, Z.,Wang, Y.,Wang, X.,Qin, C.F.
Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2.
Natl Sci Rev, 8:nwaa297-nwaa297, 2021

PubMed Abstract: Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2. Halting these steps can cure COVID-19. Here we have identified and characterized a potent human monoclonal antibody, HB27, that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations. Remarkably, HB27 can also prevent SARS-CoV-2 membrane fusion. Consequently, a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models. Rhesus macaques showed no obvious adverse events when administrated with 10 times the effective dose of HB27. Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor. Binding of the antibody also restrains any further conformational changes of the receptor binding domain, possibly interfering with progression from the prefusion to the postfusion stage. These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19.

PubMed: 34676096
DOI: 10.1093/nsr/nwaa297

実験手法

ELECTRON MICROSCOPY (3.5 Å)