7CXN

Architecture of a SARS-CoV-2 mini replication and transcription complex

7CXN の概要

• エントリーDOI: 10.2210/pdb7cxn/pdb
• EMDBエントリー: 30493
• 分子名称: RNA-directed RNA polymerase, Non-structural protein 8, Non-structural protein 7, ... (8 entities in total)
• 機能のキーワード: sars-cov-2, rtc, viral protein-rna complex, viral protein/rna
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 324119.80

構造登録者

Yan, L.,Zhang, Y.,Ge, J.,Zheng, L.,Gao, Y.,Wang, T.,Jia, Z.,Wang, H.,Huang, Y.,Li, M.,Wang, Q.,Rao, Z.,Lou, Z. (登録日: 2020-09-02, 公開日: 2020-11-04, 最終更新日: 2025-06-25)

主引用文献

Yan, L.,Zhang, Y.,Ge, J.,Zheng, L.,Gao, Y.,Wang, T.,Jia, Z.,Wang, H.,Huang, Y.,Li, M.,Wang, Q.,Rao, Z.,Lou, Z.
Architecture of a SARS-CoV-2 mini replication and transcription complex.
Nat Commun, 11:5874-5874, 2020

PubMed Abstract: Non-structural proteins (nsp) constitute the SARS-CoV-2 replication and transcription complex (RTC) to play a pivotal role in the virus life cycle. Here we determine the atomic structure of a SARS-CoV-2 mini RTC, assembled by viral RNA-dependent RNA polymerase (RdRp, nsp12) with a template-primer RNA, nsp7 and nsp8, and two helicase molecules (nsp13-1 and nsp13-2), by cryo-electron microscopy. Two groups of mini RTCs with different conformations of nsp13-1 are identified. In both of them, nsp13-1 stabilizes overall architecture of the mini RTC by contacting with nsp13-2, which anchors the 5'-extension of RNA template, as well as interacting with nsp7-nsp8-nsp12-RNA. Orientation shifts of nsp13-1 results in its variable interactions with other components in two forms of mini RTC. The mutations on nsp13-1:nsp12 and nsp13-1:nsp13-2 interfaces prohibit the enhancement of helicase activity achieved by mini RTCs. These results provide an insight into how helicase couples with polymerase to facilitate its function in virus replication and transcription.

PubMed: 33208736
DOI: 10.1038/s41467-020-19770-1

実験手法

ELECTRON MICROSCOPY (3.84 Å)