7CU1

CRYSTAL STRUCTURE OF STREPTOMYCES ALBOGRISEOLUS FLAVIN-DEPENDENT TRYPTOPHAN 6-HALOGENASE (THAL) IN COMPLEX WITH FAD and AMP

Summary for 7CU1

• Entry DOI: 10.2210/pdb7cu1/pdb
• Descriptor: Tryptophan 6-halogenase, FLAVIN-ADENINE DINUCLEOTIDE, ADENOSINE MONOPHOSPHATE, ... (4 entities in total)
• Functional Keywords: flavin monooxygenase, flavin-dependent tryptophan 6-halogenase, oxidoreductase
• Biological source: Streptomyces albogriseolus
• Total number of polymer chains: 2
• Total formula weight: 125659.13

Authors

Chitnumsub, P.,Jaruwat, A.,Phintha, A.,Chaiyen, P. (deposition date: 2020-08-20, release date: 2020-11-25, Last modification date: 2023-11-29)

Primary citation

Phintha, A.,Prakinee, K.,Jaruwat, A.,Lawan, N.,Visitsatthawong, S.,Kantiwiriyawanitch, C.,Songsungthong, W.,Trisrivirat, D.,Chenprakhon, P.,Mulholland, A.,van Pee, K.H.,Chitnumsub, P.,Chaiyen, P.
Dissecting the low catalytic capability of flavin-dependent halogenases.
J.Biol.Chem., 296:100068-100068, 2020

PubMed Abstract: Although flavin-dependent halogenases (FDHs) are attractive biocatalysts, their practical applications are limited because of their low catalytic efficiency. Here, we investigated the reaction mechanisms and structures of tryptophan 6-halogenase (Thal) from Streptomyces albogriseolus using stopped-flow, rapid-quench flow, quantum/mechanics molecular mechanics calculations, crystallography, and detection of intermediate (hypohalous acid [HOX]) liberation. We found that the key flavin intermediate, C4a-hydroperoxyflavin (C4aOOH-FAD), formed by Thal and other FDHs (tryptophan 7-halogenase [PrnA] and tryptophan 5-halogenase [PyrH]), can react with I, Br, and Cl but not F to form C4a-hydroxyflavin and HOX. Our experiments revealed that I reacts with C4aOOH-FAD the fastest with the lowest energy barrier and have shown for the first time that a significant amount of the HOX formed leaks out as free HOX. This leakage is probably a major cause of low product coupling ratios in all FDHs. Site-saturation mutagenesis of Lys79 showed that changing Lys79 to any other amino acid resulted in an inactive enzyme. However, the levels of liberated HOX of these variants are all similar, implying that Lys79 probably does not form a chloramine or bromamine intermediate as previously proposed. Computational calculations revealed that Lys79 has an abnormally lower pKa compared with other Lys residues, implying that the catalytic Lys may act as a proton donor in catalysis. Analysis of new X-ray structures of Thal also explains why premixing of FDHs with reduced flavin adenine dinucleotide generally results in abolishment of C4aOOH-FAD formation. These findings reveal the hidden factors restricting FDHs capability which should be useful for future development of FDHs applications.

PubMed: 33465708
DOI: 10.1074/jbc.RA120.016004

Experimental method

X-RAY DIFFRACTION (1.91 Å)