7CS6

IiPLR1 with NADP+ and (-)lariciresinol

Summary for 7CS6

• Entry DOI: 10.2210/pdb7cs6/pdb
• Descriptor: Pinoresinol-lariciresinol reductase, 4-[[(3S,4S,5R)-4-(hydroxymethyl)-5-(3-methoxy-4-oxidanyl-phenyl)oxolan-3-yl]methyl]-2-methoxy-phenol, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• Functional Keywords: iiplr1, nadp+, lariciresinol, plant protein, oxidoreductase
• Biological source: Isatis tinctoria (Dyer's woad)
• Total number of polymer chains: 6
• Total formula weight: 220610.45

Authors

Shao, K.,Zhang, P. (deposition date: 2020-08-14, release date: 2021-06-09, Last modification date: 2023-11-29)

Primary citation

Xiao, Y.,Shao, K.,Zhou, J.,Wang, L.,Ma, X.,Wu, D.,Yang, Y.,Chen, J.,Feng, J.,Qiu, S.,Lv, Z.,Zhang, L.,Zhang, P.,Chen, W.
Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases.
Nat Commun, 12:2828-2828, 2021

PubMed Abstract: Pinoresinol-lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis after the initial dimerization of two monolignols, and this represents the entry point for the synthesis of 8-8' lignans and contributes greatly to their structural diversity. Of particular interest has been the determination of how differing substrate specificities are achieved with these enzymes. Here, we present crystal structures of IiPLR1 from Isatis indigotica and pinoresinol reductases (PrRs) AtPrR1 and AtPrR2 from Arabidopsis thaliana, in the apo, substrate-bound and product-bound states. Each structure contains a head-to-tail homodimer, and the catalytic pocket comprises structural elements from both monomers. β4 loop covers the top of the pocket, and residue 98 from the loop governs catalytic specificity. The substrate specificities of IiPLR1 and AtPrR2 can be switched via structure-guided mutagenesis. Our study provides insight into the molecular mechanism underlying the substrate specificity of PLRs/PrRs and suggests an efficient strategy for the large-scale commercial production of the pharmaceutically valuable compound lariciresinol.

PubMed: 33990581
DOI: 10.1038/s41467-021-23095-y

Experimental method

X-RAY DIFFRACTION (2.20139658355 Å)