7CPU

Cryo-EM structure of 80S ribosome from mouse kidney

This is a non-PDB format compatible entry.

Summary for 7CPU

• Entry DOI: 10.2210/pdb7cpu/pdb
• EMDB information: 30432
• Descriptor: 60S ribosomal protein L8, 60S ribosomal protein L9, 60S ribosomal protein L10, ... (81 entities in total)
• Functional Keywords: 80s mouse ribosome, protein translation, rpl39, rpl39l, kidney ribosome, ribosome
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 78
• Total formula weight: 3680083.95

Authors

Huo, Y.G.,He, X.,Jiang, T.,Qin, Y.,Guo, X.J.,Sha, J.H. (deposition date: 2020-08-08, release date: 2022-02-02, Last modification date: 2024-05-29)

Primary citation

Li, H.,Huo, Y.,He, X.,Yao, L.,Zhang, H.,Cui, Y.,Xiao, H.,Xie, W.,Zhang, D.,Wang, Y.,Zhang, S.,Tu, H.,Cheng, Y.,Guo, Y.,Cao, X.,Zhu, Y.,Jiang, T.,Guo, X.,Qin, Y.,Sha, J.
A male germ-cell-specific ribosome controls male fertility.
Nature, 612:725-731, 2022

PubMed Abstract: Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis. Male germ cell development involves complex translational regulation during sperm formation. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, Ribosome, that is assembled with the male germ-cell-specific protein RPL39L, the paralogue of core ribosome (Ribosome) protein RPL39. Deletion of Ribosome in mice causes defective sperm formation, resulting in substantially reduced fertility. Our comparison of single-particle cryo-electron microscopy structures of ribosomes from mouse kidneys and testes indicates that Ribosome features a ribosomal polypeptide exit tunnel of distinct size and charge states compared with Ribosome. Ribosome predominantly cotranslationally regulates the folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm. Moreover, we found that specialized functions of Ribosome were not replaceable by Ribosome. Taken together, identification of this sperm-specific ribosome should greatly expand our understanding of ribosome function and tissue-specific regulation of protein expression pattern in mammals.

PubMed: 36517592
DOI: 10.1038/s41586-022-05508-0

Experimental method

ELECTRON MICROSCOPY (2.82 Å)