7CPU

Cryo-EM structure of 80S ribosome from mouse kidney

これはPDB形式変換不可エントリーです。

7CPU の概要

• エントリーDOI: 10.2210/pdb7cpu/pdb
• EMDBエントリー: 30432
• 分子名称: 60S ribosomal protein L8, 60S ribosomal protein L9, 60S ribosomal protein L10, ... (81 entities in total)
• 機能のキーワード: 80s mouse ribosome, protein translation, rpl39, rpl39l, kidney ribosome, ribosome
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 78
• 化学式量合計: 3680083.95

構造登録者

Huo, Y.G.,He, X.,Jiang, T.,Qin, Y.,Guo, X.J.,Sha, J.H. (登録日: 2020-08-08, 公開日: 2022-02-02, 最終更新日: 2024-05-29)

主引用文献

Li, H.,Huo, Y.,He, X.,Yao, L.,Zhang, H.,Cui, Y.,Xiao, H.,Xie, W.,Zhang, D.,Wang, Y.,Zhang, S.,Tu, H.,Cheng, Y.,Guo, Y.,Cao, X.,Zhu, Y.,Jiang, T.,Guo, X.,Qin, Y.,Sha, J.
A male germ-cell-specific ribosome controls male fertility.
Nature, 612:725-731, 2022

PubMed Abstract: Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis. Male germ cell development involves complex translational regulation during sperm formation. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, Ribosome, that is assembled with the male germ-cell-specific protein RPL39L, the paralogue of core ribosome (Ribosome) protein RPL39. Deletion of Ribosome in mice causes defective sperm formation, resulting in substantially reduced fertility. Our comparison of single-particle cryo-electron microscopy structures of ribosomes from mouse kidneys and testes indicates that Ribosome features a ribosomal polypeptide exit tunnel of distinct size and charge states compared with Ribosome. Ribosome predominantly cotranslationally regulates the folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm. Moreover, we found that specialized functions of Ribosome were not replaceable by Ribosome. Taken together, identification of this sperm-specific ribosome should greatly expand our understanding of ribosome function and tissue-specific regulation of protein expression pattern in mammals.

PubMed: 36517592
DOI: 10.1038/s41586-022-05508-0

実験手法

ELECTRON MICROSCOPY (2.82 Å)