7CPC

His-Mediated Reversible Self-assembly of Ferritin Nanocage with Ni binding

7CPC の概要

• エントリーDOI: 10.2210/pdb7cpc/pdb
• 分子名称: Ferritin, NICKEL (II) ION, FE (III) ION, ... (4 entities in total)
• 機能のキーワード: ferritin, assembly, ni binding, metal binding protein
• 由来する生物種: Penaeus japonicus (Kuruma prawn)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 119097.52

構造登録者

Gu, C.,Zhang, T.,Zhao, G. (登録日: 2020-08-06, 公開日: 2021-06-16, 最終更新日: 2023-11-29)

主引用文献

Gu, C.,Zhang, T.,Lv, C.,Liu, Y.,Wang, Y.,Zhao, G.
His-Mediated Reversible Self-Assembly of Ferritin Nanocages through Two Different Switches for Encapsulation of Cargo Molecules.
Acs Nano, 2020

PubMed Abstract: Protein nanocages represent a class of nanovehicles for a variety of applications. However, precise manipulation of self-assembly behavior of these protein nanocages in response to multiple external stimuli for custom-tailored applications remains challenging. Herein, we established a simple but effective strategy for controlling protein nanocage self-assembly that combines a dual property of His motifs (their significantly pH-dependent protonation state and their capacity to coordinate with transition metals) with its high symmetry. With this strategy, we enabled two different ferritin nanocages to disassemble into protein tetramers under neutral solution by introducing His motifs at the 4-fold channel interfaces. Notably, these tetramers are able to self-assemble into ferritin-like protein nanocages in response to multiple external stimuli such as transition metal ions and pH, and , indicative of a reversible self-assembly process. Furthermore, such His-mediated reversible protein self-assembly has been explored for encapsulation of bioactive cargo molecules within these reconstituted protein nanocages with higher loading efficiency under milder conditions as compared to the reported acid denaturation encapsulation method for ferritin.

PubMed: 33197176
DOI: 10.1021/acsnano.0c06670

実験手法

X-RAY DIFFRACTION (2.801 Å)