7CM1

Neuraminidase from the Wuhan Asiatic toad influenza virus

Summary for 7CM1

• Entry DOI: 10.2210/pdb7cm1/pdb
• Descriptor: Neuraminidase, CALCIUM ION, (3R,4R,5S)-4-(acetylamino)-5-amino-3-(pentan-3-yloxy)cyclohex-1-ene-1-carboxylic acid, ... (4 entities in total)
• Functional Keywords: lattice-translocation defects ltd neuraminidase na, viral protein, hydrolase
• Biological source: Wuhan asiatic toad influenza virus
• Total number of polymer chains: 2
• Total formula weight: 84620.23

Authors

Wang, J. (deposition date: 2020-07-23, release date: 2020-11-18, Last modification date: 2024-10-23)

Primary citation

Li, L.,Dai, S.,Gao, G.F.,Wang, J.
Lattice-translocation defects in specific crystals of the catalytic head domain of influenza neuraminidase.
Acta Crystallogr D Struct Biol, 76:1057-1064, 2020

PubMed Abstract: Neuraminidase (NA) inhibitors are one of the two major classes of antivirals available for the treatment and prevention of influenza. X-ray crystal structure determination of NA head domains and their complexes with various inhibitors are of importance for the design and optimization of anti-influenza drugs. However, the globular tetrameric properties of NA head domains may produce crystals with pathological imperfections, lattice-translocation defects, making structure determination no longer straightforward. In this report, using a crystal of the NA head domain from the Wuhan Asiatic toad influenza virus as an example, the identification and solution of this type of crystal pathology are presented. Furthermore, its underlying mechanism of formation is explored.

PubMed: 33135677
DOI: 10.1107/S2059798320011869

Experimental method

X-RAY DIFFRACTION (2.14 Å)