7CLY

Structure of the DOCK8 DHR-1 domain crystallized with di-C8-phosphatidylinositol-(4,5)-bisphosphate

7CLY の概要

• エントリーDOI: 10.2210/pdb7cly/pdb
• 関連するPDBエントリー: 7CLX
• 分子名称: Dedicator of cytokinesis protein 8 (2 entities in total)
• 機能のキーワード: dock, guanine nucleotide exchange factor, gtpase, cdc42, membrane, di-c8-pi(4, 5)p2, phosphoinositide, signaling protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21482.45

構造登録者

Kukimoto-Niino, M.,Shirouzu, M.,Yokoyama, S.,Fukui, Y.,Uruno, T. (登録日: 2020-07-22, 公開日: 2021-02-10, 最終更新日: 2023-11-29)

主引用文献

Sakurai, T.,Kukimoto-Niino, M.,Kunimura, K.,Yamane, N.,Sakata, D.,Aihara, R.,Yasuda, T.,Yokoyama, S.,Shirouzu, M.,Fukui, Y.,Uruno, T.
A conserved PI(4,5)P2-binding domain is critical for immune regulatory function of DOCK8.
Life Sci Alliance, 4:-, 2021

PubMed Abstract: DOCK8 is a Cdc42-specific guanine-nucleotide exchange factor that is essential for development and functions of various subsets of leukocytes in innate and acquired immune responses. Although DOCK8 plays a critical role in spatial control of Cdc42 activity during interstitial leukocyte migration, the mechanism remains unclear. We show that the DOCK homology region (DHR)-1 domain of DOCK8 binds specifically to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and is required for its recruitment to the plasma membrane. Structural and biochemical analyses reveal that DOCK8 DHR-1 domain consists of a C2 domain-like core with loops creating the upper surface pocket, where three basic residues are located for stereospecific recognition of phosphoinositides. Substitution of the two basic residues, K576 and R581, with alanine abolished PI(4,5)P2 binding in vitro, ablated the ability of DOCK8 to activate Cdc42 and support leukocyte migration in three-dimensional collagen gels. Dendritic cells carrying the mutation exhibited defective interstitial migration in vivo. Thus, our study uncovers a critical role of DOCK8 in coupling PI(4,5)P2 signaling with Cdc42 activation for immune regulation.

PubMed: 33574036
DOI: 10.26508/lsa.202000873

実験手法

X-RAY DIFFRACTION (1.432 Å)