7CJ5

Crystal structure of homo dimeric D-allulose 3-epimerase from Methylomonas sp. in complex with D-fructose

7CJ5 の概要

• エントリーDOI: 10.2210/pdb7cj5/pdb
• 関連するPDBエントリー: 7CJ4
• 分子名称: Epimerase, MANGANESE (II) ION, D-fructose, ... (6 entities in total)
• 機能のキーワード: epimerase, tim barrel, isomerase
• 由来する生物種: Methylomonas sp. DH-1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65313.49

構造登録者

Yoshida, H.,Yoshihara, A.,Kamitori, S. (登録日: 2020-07-09, 公開日: 2021-04-28, 最終更新日: 2023-11-29)

主引用文献

Yoshida, H.,Yoshihara, A.,Kato, S.,Mochizuki, S.,Akimitsu, K.,Izumori, K.,Kamitori, S.
Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.
Febs Open Bio, 11:1621-1637, 2021

PubMed Abstract: d-Allulose has potential as a low-calorie sweetener which can suppress fat accumulation. Several enzymes capable of d-allulose production have been isolated, including d-tagatose 3-epimerases. Here, we report the isolation of a novel protein from Methylomonas sp. expected to be a putative enzyme based on sequence similarity to ketose 3-epimerase. The synthesized gene encoding the deduced ketose 3-epimerase was expressed as a recombinant enzyme in Escherichia coli, and it exhibited the highest enzymatic activity toward l-ribulose, followed by d-ribulose and d-allulose. The X-ray structure analysis of l-ribulose 3-epimerase from Methylomonas sp. (MetLRE) revealed a homodimeric enzyme, the first reported structure of dimeric l-ribulose 3-epimerase. The monomeric structure of MetLRE is similar to that of homotetrameric l-ribulose 3-epimerases, but the short C-terminal α-helix of MetLRE is unique and different from those of known l-ribulose 3 epimerases. The length of the C-terminal α-helix was thought to be involved in tetramerization and increasing stability; however, the addition of residues to MetLRE at the C terminus did not lead to tetramer formation. MetLRE is the first dimeric l-ribulose 3-epimerase identified to exhibit high relative activity toward d-allulose.

PubMed: 33838083
DOI: 10.1002/2211-5463.13159

実験手法

X-RAY DIFFRACTION (1.8 Å)