7CEB

Crystal structure of alpha6beta1 integrin headpiece

7CEB の概要

• エントリーDOI: 10.2210/pdb7ceb/pdb
• 分子名称: Integrin alpha-6, Integrin beta-1, TS2/16 VH(S112C)-SARAH, ... (8 entities in total)
• 機能のキーワード: integrin, fv-clasp, laminin, cell adhesion, cell adhesion-immune system complex, cell adhesion/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 159533.55

構造登録者

Arimori, T.,Takagi, J. (登録日: 2020-06-22, 公開日: 2021-06-23, 最終更新日: 2024-10-30)

主引用文献

Arimori, T.,Miyazaki, N.,Mihara, E.,Takizawa, M.,Taniguchi, Y.,Cabanas, C.,Sekiguchi, K.,Takagi, J.
Structural mechanism of laminin recognition by integrin.
Nat Commun, 12:4012-4012, 2021

PubMed Abstract: Recognition of laminin by integrin receptors is central to the epithelial cell adhesion to basement membrane, but the structural background of this molecular interaction remained elusive. Here, we report the structures of the prototypic laminin receptor α6β1 integrin alone and in complex with three-chain laminin-511 fragment determined via crystallography and cryo-electron microscopy, respectively. The laminin-integrin interface is made up of several binding sites located on all five subunits, with the laminin γ1 chain C-terminal portion providing focal interaction using two carboxylate anchor points to bridge metal-ion dependent adhesion site of integrin β1 subunit and Asn189 of integrin α6 subunit. Laminin α5 chain also contributes to the affinity and specificity by making electrostatic interactions with large surface on the β-propeller domain of α6, part of which comprises an alternatively spliced X1 region. The propeller sheet corresponding to this region shows unusually high mobility, suggesting its unique role in ligand capture.

PubMed: 34188035
DOI: 10.1038/s41467-021-24184-8

実験手法

X-RAY DIFFRACTION (2.89 Å)