7CE1

Complex STRUCTURE OF TRANSCRIPTION FACTOR SghR with its COGNATE DNA

7CE1 の概要

• エントリーDOI: 10.2210/pdb7ce1/pdb
• 分子名称: LacI-type transcription factor, promoter DNA (3 entities in total)
• 機能のキーワード: agrobacterium infection; plant-microbe interaction; laci rep sucrose, transcription
• 由来する生物種: Agrobacterium tumefaciens A6; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 521796.20

構造登録者

Ye, F.Z.,Wang, C.,Yan, X.F.,Zhang, L.H.,Gao, Y.G. (登録日: 2020-06-21, 公開日: 2020-07-15, 最終更新日: 2023-11-29)

主引用文献

Ye, F.,Wang, C.,Fu, Q.,Yan, X.F.,Bharath, S.R.,Casanas, A.,Wang, M.,Song, H.,Zhang, L.H.,Gao, Y.G.
Structural basis of a novel repressor, SghR, controllingAgrobacteriuminfection by cross-talking to plants.
J.Biol.Chem., 295:12290-12304, 2020

PubMed Abstract: infects various plants and causes crown gall diseases involving temporal expression of virulence factors. SghA is a newly identified virulence factor enzymatically releasing salicylic acid from its glucoside conjugate and controlling plant tumor development. Here, we report the structural basis of SghR, a LacI-type transcription factor highly conserved in family, regulating the expression of SghA and involved in tumorigenesis. We identified and characterized the binding site of SghR on the promoter region of and then determined the crystal structures of apo-SghR, SghR complexed with its operator DNA, and ligand sucrose, respectively. These results provide detailed insights into how SghR recognizes its cognate DNA and shed a mechanistic light on how sucrose attenuates the affinity of SghR with DNA to modulate the expression of SghA. Given the important role of SghR in mediating the signaling cross-talk during infection, our results pave the way for structure-based inducer analog design, which has potential applications for agricultural industry.

PubMed: 32651231
DOI: 10.1074/jbc.RA120.012908

実験手法

X-RAY DIFFRACTION (3.2 Å)