7CCI

Acinetobacter baumannii response regulator AdeR with disordered N terminus

7CCI の概要

• エントリーDOI: 10.2210/pdb7cci/pdb
• 分子名称: AdeR, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: acinetobacter baumannii, response regulator, ader, dna binding protein, receiver domain
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32930.63

構造登録者

Wen, Y. (登録日: 2020-06-17, 公開日: 2021-06-23, 最終更新日: 2023-11-29)

主引用文献

Ouyang, Z.,Zheng, F.,Zhu, L.,Felix, J.,Wu, D.,Wu, K.,Gutsche, I.,Wu, Y.,Hwang, P.M.,She, J.,Wen, Y.
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.
Iscience, 24:102476-102476, 2021

PubMed Abstract: Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The two-component regulatory system AdeRS is made up of a sensor histidine kinase AdeS and a cognate response regulator AdeR, which together reduce repression of the multidrug-resistant efflux pump AdeABC. Herein we demonstrate that an N-terminal intrinsically disordered tail in AdeR is important for the upregulation of expression, although it greatly increases the susceptibility of AdeR to proteasome-mediated degradation. We also show that AdeS assembles into a hexameric state that is necessary for its full histidine kinase activity, which appears to occur via autophosphorylation. Taken together, this study demonstrates new structural mechanisms through which two-component systems can transduce environmental signals to impact gene expression and enlightens new potential antimicrobial approach by targeting two-component regulatory systems.

PubMed: 34113820
DOI: 10.1016/j.isci.2021.102476

実験手法

X-RAY DIFFRACTION (1.65 Å)