7CCH

Acinetobacter baumannii histidine kinase AdeS

Summary for 7CCH

• Entry DOI: 10.2210/pdb7cch/pdb
• Descriptor: AdeS (1 entity in total)
• Functional Keywords: two-component regulatory system, acinetobacter baumannii, histidine kinase, ades, multidrug resistance, signaling protein
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 1
• Total formula weight: 25908.74

Authors

Wen, Y.,Felix, J. (deposition date: 2020-06-17, release date: 2021-06-23, Last modification date: 2023-11-29)

Primary citation

Ouyang, Z.,Zheng, F.,Zhu, L.,Felix, J.,Wu, D.,Wu, K.,Gutsche, I.,Wu, Y.,Hwang, P.M.,She, J.,Wen, Y.
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.
Iscience, 24:102476-102476, 2021

PubMed Abstract: Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The two-component regulatory system AdeRS is made up of a sensor histidine kinase AdeS and a cognate response regulator AdeR, which together reduce repression of the multidrug-resistant efflux pump AdeABC. Herein we demonstrate that an N-terminal intrinsically disordered tail in AdeR is important for the upregulation of expression, although it greatly increases the susceptibility of AdeR to proteasome-mediated degradation. We also show that AdeS assembles into a hexameric state that is necessary for its full histidine kinase activity, which appears to occur via autophosphorylation. Taken together, this study demonstrates new structural mechanisms through which two-component systems can transduce environmental signals to impact gene expression and enlightens new potential antimicrobial approach by targeting two-component regulatory systems.

PubMed: 34113820
DOI: 10.1016/j.isci.2021.102476

Experimental method

X-RAY DIFFRACTION (2.848 Å)