7C9P

Crystal structure of rice histone-fold dimer GHD8/OsNF-YC2

7C9P の概要

• エントリーDOI: 10.2210/pdb7c9p/pdb
• 分子名称: Nuclear transcription factor Y subunit B-11, Nuclear transcription factor Y subunit C-2 (3 entities in total)
• 機能のキーワード: photoperiodic flowering, cct family, nf-cct complex, specific dna recognition, transcription
• 由来する生物種: Oryza sativa Japonica Group (Rice); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45514.13

構造登録者

Shen, C.,Liu, H.,Guan, Z.,Xing, Y.,Yin, P. (登録日: 2020-06-06, 公開日: 2020-09-09, 最終更新日: 2023-11-29)

主引用文献

Shen, C.,Liu, H.,Guan, Z.,Yan, J.,Zheng, T.,Yan, W.,Wu, C.,Zhang, Q.,Yin, P.,Xing, Y.
Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.
Plant Cell, 32:3469-3484, 2020

PubMed Abstract: CONSTANS, CONSTANS-LIKE, and TIMING OF CAB EXPRESSION1 (CCT) domain-containing proteins are a large family unique to plants. They transcriptionally regulate photoperiodic flowering, circadian rhythms, vernalization, and other related processes. Through their CCT domains, CONSTANS and HEADING DATE1 (HD1) coordinate with the NUCLEAR FACTOR Y (NF-Y) B/C dimer to specifically target a conserved 'CCACA' motif within the promoters of their target genes. However, the mechanism underlying DNA recognition by the CCT domain remains unclear. Here we determined the crystal structures of the rice () NF-YB/YC dimer and the florigen gene ()-bound HD1/NF-YB/YC trimer with resolutions of 2.0 Å and 2.55 Å, respectively. The CCT domain of HD1 displays an elongated structure containing two α-helices and two loops, tethering to the NF-YB/YC dimer. Helix α2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide insight into the network between the CCT proteins and NF-Y subunits, but also offer potential approaches for improving productivity and global adaptability of crops by manipulating florigen expression.

PubMed: 32843433
DOI: 10.1105/tpc.20.00067

実験手法

X-RAY DIFFRACTION (2 Å)