7C7D

Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3

7C7D の概要

• エントリーDOI: 10.2210/pdb7c7d/pdb
• 分子名称: alpha-1,3-glucanase, CALCIUM ION, PENTAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: glycosidase, hydrolase
• 由来する生物種: Streptomyces thermodiastaticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 126870.70

構造登録者

Itoh, T.,Panti, N.,Toyotake, Y.,Hayashi, J.,Suyotha, W.,Yano, S.,Wakayama, M.,Hibi, T. (登録日: 2020-05-25, 公開日: 2020-11-11, 最終更新日: 2024-11-06)

主引用文献

Itoh, T.,Panti, N.,Hayashi, J.,Toyotake, Y.,Matsui, D.,Yano, S.,Wakayama, M.,Hibi, T.
Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3.
Biochem.Biophys.Res.Commun., 533:1170-1176, 2020

PubMed Abstract: α-1,3-Glucan is a homopolymer composed of D-glucose (Glc) and it is an extracellular polysaccharide found in dental plaque due to Streptococcus species. α-1,3-Glucanase from Streptomyces thermodiastaticus strain HF3-3 (Agl-ST) has been identified as a thermostable α-1,3-glucanase, which is classified into glycoside hydrolase family 87 (GH87) and specifically hydrolyzes α-1,3-glucan with an endo-action. The enzyme has a potential to inhibit the production of dental plaque and to be used for biotechnological applications. Here we show the structure of the catalytic unit of Agl-ST determined at 1.16 Å resolution using X-ray crystallography. The catalytic unit is composed of two modules, a β-sandwich fold module, and a right-handed β-helix fold module, which resembles other structural characterized GH87 enzymes from Bacillus circulans str. KA-304 and Paenibacillus glycanilyticus str. FH11, with moderate sequence identities between each other (approximately 27% between the catalytic units). However, Agl-ST is smaller in size and more thermally stable than the others. A disulfide bond that anchors the C-terminal coil of the β-helix fold, which is expected to contribute to thermal stability only exists in the catalytic unit of Agl-ST.

PubMed: 33041007
DOI: 10.1016/j.bbrc.2020.09.133

実験手法

X-RAY DIFFRACTION (1.16 Å)