7C6O

Catalytic Subunit of Cobaltochelatase from Mycobacterium tuberculosis

7C6O の概要

• エントリーDOI: 10.2210/pdb7c6o/pdb
• 分子名称: Cobalamin biosynthesis protein CobN, GLYCEROL, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: co-chelatase, metal binding protein, ligase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 133573.15

構造登録者

Zhang, J.,Liu, L. (登録日: 2020-05-22, 公開日: 2020-12-02, 最終更新日: 2023-11-29)

主引用文献

Zhang, J.H.,Yuan, H.,Wang, X.,Dai, H.E.,Zhang, M.,Liu, L.
Crystal structure of the large subunit of cobaltochelatase from Mycobacterium tuberculosis.
Proteins, 89:462-467, 2021

PubMed Abstract: Cobaltochelatase in aerobic cobalamin biosynthesis is a complex composed of three subunits. The large subunit CobN is a 140-kDa protein and is homologous to the ChlH subunit of magnesium chelatase. Previously we have reported the 2.5-Å structure of a cyanobacterial ChlH. Here we present the 1.8-Å structure of CobN from Mycobacterium tuberculosis. The overall structure of CobN and ChlH is similar, but significant difference occurs in the head domain. Structural comparison of domains between the two proteins unravels candidate regions for substrate binding and helps to locate a triad of residues that may be essential for metal ion binding.

PubMed: 33210347
DOI: 10.1002/prot.26023

実験手法

X-RAY DIFFRACTION (1.801 Å)