7C1S

Crystal structure of the starter condensation domain of rhizomide synthetase RzmA mutant H140A/R148A in complex with C8-CoA and Leu-SNAC

Summary for 7C1S

• Entry DOI: 10.2210/pdb7c1s/pdb
• Related: 7C1H 7C1K 7C1L 7C1P 7C1R
• Descriptor: Non-ribosomal peptide synthetase modules, OCTANOYL-COENZYME A, S-(2-acetamidoethyl) (2S)-2-azanyl-4-methyl-pentanethioate, ... (4 entities in total)
• Functional Keywords: nonribosomal peptide synthesis, rzma-cs, starter condensation (cs) domains, c8-coa, leu-snac, biosynthetic protein
• Biological source: Paraburkholderia rhizoxinica HKI 454
• Total number of polymer chains: 1
• Total formula weight: 49544.86

Authors

Zhong, L.,Diao, X.,Zhang, N.,Li, F.W.,Zhou, H.B.,Chen, H.N.,Ren, X.,Zhang, Y.,Wu, D.,Bian, X. (deposition date: 2020-05-05, release date: 2020-11-25, Last modification date: 2023-11-29)

Primary citation

Zhong, L.,Diao, X.,Zhang, N.,Li, F.,Zhou, H.,Chen, H.,Bai, X.,Ren, X.,Zhang, Y.,Wu, D.,Bian, X.
Engineering and elucidation of the lipoinitiation process in nonribosomal peptide biosynthesis.
Nat Commun, 12:296-296, 2021

PubMed Abstract: Nonribosomal peptide synthetases containing starter condensation domains direct the biosynthesis of nonribosomal lipopeptides, which generally exhibit wide bioactivities. The acyl chain has strong impacts on bioactivity and toxicity, but the lack of an in-depth understanding of starter condensation domain-mediated lipoinitiation limits the bioengineering of NRPSs to obtain novel derivatives with desired acyl chains. Here, we show that the acyl chains of the lipopeptides rhizomide, holrhizin, and glidobactin were modified by engineering the starter condensation domain, suggesting a workable approach to change the acyl chain. Based on the structure of the mutated starter condensation domain of rhizomide biosynthetic enzyme RzmA in complex with octanoyl-CoA and related point mutation experiments, we identify a set of residues responsible for the selectivity of substrate acyl chains and extend the acyl chains from acetyl to palmitoyl. Furthermore, we illustrate three possible conformational states of starter condensation domains during the reaction cycle of the lipoinitiation process. Our studies provide further insights into the mechanism of lipoinitiation and the engineering of nonribosomal peptide synthetases.

PubMed: 33436600
DOI: 10.1038/s41467-020-20548-8

Experimental method

X-RAY DIFFRACTION (2.586 Å)