7C0K

Crystal structure of a dinucleotide-binding protein of ABC transporter endogenously bound to uridylyl-3'-5'-phospho-guanosine (Form II)

7C0K の概要

• エントリーDOI: 10.2210/pdb7c0k/pdb
• 分子名称: Sugar ABC transporter, periplasmic sugar-binding protein, 2-HYDROXY BUTANE-1,4-DIOL, TETRAETHYLENE GLYCOL, ... (15 entities in total)
• 機能のキーワード: c-di-gmp/amp, substrate-binding protein, thermus thermophilus, trna synthesis and/or modification, venus fly-trap mechanism, ugpb, transport protein
• 由来する生物種: Thermus thermophilus HB8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93382.47

構造登録者

Kanaujia, S.P.,Chandravanshi, M. (登録日: 2020-05-01, 公開日: 2021-03-10, 最終更新日: 2024-10-23)

主引用文献

Chandravanshi, M.,Samanta, R.,Kanaujia, S.P.
Structural and thermodynamic insights into the novel dinucleotide-binding protein of ABC transporter unveils its moonlighting function.
Febs J., 288:4614-4636, 2021

PubMed Abstract: Substrate (or solute)-binding proteins (SBPs) selectively bind the target ligands and deliver them to the ATP-binding cassette (ABC) transport system for their translocation. Irrespective of the different types of ligands, SBPs are structurally conserved. A wealth of structural details of SBPs bound to different types of ligands and the physiological basis of their import are available; however, the uptake mechanism of nucleotides is still deficient. In this study, we elucidated the structural details of an SBP endogenously bound to a novel ligand, a derivative of uridylyl-3'-5'-phospho-guanosine (U3G); thus, we named it a U3G-binding protein (U3GBP). To the best of our knowledge, this is the first report of U3G (and a dinucleotide) binding to the SBP of ABC transport system, and thus, U3GBP is classified as a first member of subcluster D-I SBPs. Thermodynamic data also suggest that U3GBP can bind phospholipid precursor sn-glycerophosphocholine (GPC) at a site other than the active site. Moreover, a combination of mutagenic and structural information reveals that the protein U3GBP follows the well-known 'Venus Fly-trap' mechanism for dinucleotide binding. DATABASES: Structural data are available in RCSB Protein Data Bank under the accession number(s) 7C0F, 7C0K, 7C0L, 7C0O, 7C0R, 7C0S, 7C0T, 7C0U, 7C0V, 7C0W, 7C0X, 7C0Y, 7C0Z, 7C14, 7C15, 7C16, 7C19, and 7C1B.

PubMed: 33599038
DOI: 10.1111/febs.15774

実験手法

X-RAY DIFFRACTION (1.8 Å)